NDT401-Da: Testing & titration of primary antibody for colorimetric immunostaining on FREE-FLOATING sections

This service includes tissue preparation, sectioning, immunolabeling with various dilutions of the primary antibody, mounting, coverslipping and labeling the slides. As a result, you will receive up to 30 immunostained tissue sections ready for microscopic observations. In addition, the experimental procedure used for the antibody titration will be provided.

Procedure: 

Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Gelatin-Coated Microscope Slides).  Subsequently, sections will be processed free-floating for immunostaining with the specific antibody at various dilutions according to the avidin-biotin-complex (ABC) method¹.

Remarks:

• A quotation is required before placing an order.
• The investigator needs to provide both tissue and the specific antibody.

Reference:

1. Hsu S. M., Raine L. and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577-580.

NDT401-Db: Testing & titration of primary antibody for colorimetric immunostaining ON SECTIONS mounted on slides

This service includes tissue preparation, sectioning, immunolabeling with various dilutions of the primary antibody, coverslipping and labeling the slides. As a result, you will receive up to 30 immunostained tissue sections ready for microscopic observations. In addition, the experimental procedure used for the antibody titration will be provided.

Procedure:

Following cryopretection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and mounted on gelatin-coated microscope slides (cf. Gelatin-Coated Microscope Slides).  Subsequently, sections will be processed on slides for immunostaining with the specific antibody at various dilutions according to the avidin-biotin-complex (ABC) method¹.

Remarks:

• A quotation is required before placing an order.
• The investigator needs to provide both tissue and the specific antibody.

Reference:

1. Hsu S. M., Raine L. and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577-580.

NDT401-Dc: Testing & titration of primary antibody for immunofluorescence labeling on FREE-FLOATING sections

This service includes tissue preparation, sectioning, immunolabeling with various dilutions of the primary antibody, mounting, coverslipping and labeling the slides. As a result, you will receive up to 30 immunostained tissue sections ready for microscopic observations. In addition, the experimental procedure used for the antibody titration will be provided.

Procedure:

Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Gelatin-Coated Microscope Slides). Subsequently, sections will be processed free-floating for immunostaining with the specific antibody at various dilutions according to the indirect immunofluorescence method¹.

Remarks:

• A quotation is required before placing an order.
• The investigator needs to provide both tissue and the specific antibody.

Reference:

1. Coons, A.H. (1958) Fluorescent antibody methods. In J.F. Danielli (ed): General Cytochemical Methods. New York: Academic Press, pp. 399-422.

NDT401-Dd: Testing & titration of primary antibody for immunofluorescence labeling ON SECTIONS mounted on slides

This service includes tissue preparation, sectioning, immunolabeling with various dilutions of the primary antibody, coverslipping and labeling the slides. As a result, you will receive up to 30 immunostained tissue sections ready for microscopic observations. In addition, the experimental procedure used for the antibody titration will be provided.

Procedure: 

Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and mounted on gelatin-coated microscope slides (cf. Gelatin-Coated Microscope Slides).  Subsequently, sections will be processed on slides for immunostaining with the specific antibody at various dilutions according to the indirect immunofluorescence method¹. 

Remarks:

• A quotation is required before placing an order.
• The investigator needs to provide both tissue and the specific antibody.

Reference:

1. Coons, A.H. (1958) Fluorescent antibody methods. In J.F. Danielli (ed): General Cytochemical Methods. New York: Academic Press, pp. 399-422.

NDT401-De: Testing & titration of primary antibody for immunogold labeling on FREE-FLOATING sections

This service includes tissue preparation, sectioning, immunolabeling with various dilutions of the primary antibody, mounting, coverslipping and labeling the slides. As a result, you will receive up to 30 immunostained tissue sections ready for microscopic observations. In addition, the experimental procedure used for the antibody titration will be provided.

Procedure: 

Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Gelatin-Coated Microscope Slides).  Subsequently, sections will be processed free-floating for immunostaining with the specific antibody at various dilutions according to the immunogold labeling technique¹.

Remarks:

• A quotation is required before placing an order.
• The investigator needs to provide both tissue and the specific antibody.

Reference:

1. Humbel B. M., Sibon O. C. M., Stierhof Y.-D. and Schwarz H. (1995) Ultra-small gold particles and silver enhancement as a detection system in immunolabeling and In Situ Hybridization experiments. J. Histochem. Cytochem. 43, 735-737.

NDT401-Df: Testing & titration of primary antibody for immunogold labeling ON SECTIONS mounted on slides

This service includes tissue preparation, sectioning, immunolabeling with various dilutions of the primary antibody, coverslipping and labeling the slides. As a result, you will receive up to 30 immunostained tissue sections ready for microscopic observations. In addition, the experimental procedure used for the antibody titration will be provided.

Procedure: 

Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and mounted on gelatin-coated microscope slides (cf. Gelatin-Coated Microscope Slides).  Subsequently, sections will be processed on slides for immunostaining with the specific antibody at various dilutions according to the immunogold labeling technique¹.

Remarks:

• A quotation is required before placing an order.
• The investigator needs to provide both tissue and the specific antibody.

Reference:

1. Humbel B. M., Sibon O. C. M., Stierhof Y.-D. and Schwarz H. (1995) Ultra-small gold particles and silver enhancement as a detection system in immunolabeling and In Situ Hybridization experiments. J. Histochem. Cytochem. 43, 735-737.