NDT202 Cresyl violet solution, made from cresyl violet acetate certified by the BIOLOGICAL STAIN COMMISSION and with a step by step user's manual printed on the bottle, is specifically designed for easy use in all types of laboratories. This solution is formulated and extensively tested for the staining of nerve tissue. Cresyl violet imparts a violet color to nuclear chromatin and Nissl substances. This solution is excellent for visualizing neuronal cell bodies and glial nuclei. NDT202-2 is also excellent for counterstaining of immunostained sections from both neuronal and non-neuronal tissues (ref. 1-6).

H&E stain. This 5 μm paraffin section of rat intestine was stained with NDT204 FD hematoxylin (blue) and NDT203 FD eosin Y (red). 

H&E stain. This 5 μm paraffin section of mouse heart was stained with NDT204 FD hematoxylin (blue) and NDT203 FD eosin Y (red).

H&E stain. 20 μm cryostat section from the rat cortex of a stroke model was stained with NDT204 FD hematoxylin (blue) and NDT203 FD eosin Y (red). Note that damaged neurons are stained red in the cytoplasm and most of them have a condensed, irregular-shaped and darkly stained nucleus.

References

1. Bishop C, Tessmer JL, Ullrich T, Rice KC, and Walker PD: Serotonin 5-HT2A receptors underlie increased motor behaviors induced in dopamine-depleted rats by intrastriatal 5-HT2A/2C agonism. J. Pharmacol. Exp. Ther. 310: 687-694, 2004.

2. Kreipke CW, and Walker PD: NMDA Receptor blockage attenuates locomotion elicited by intrastriatal dopamine D1-receptor stimulation. Synapse  53: 28-35, 2004.

3. Nakamura NH, Rosell DR, Akama KT and McEwen BS: Estrogen and ovariectomy regulate mRNA and protein of glutamic acid decarboxylases and cation-chloride cotransporters in the adult rat hippocampus. Neuroendocrinology 80:308-323, 2004.

4. Kostich WA, Grzanna R, Lu NZ and Largent BL: Immunohistochemical visualization of corticotropin-releasing factor type 1 (CRF1) receptors in monkey brain.J. Comp. Neurol. 478:111-125, 2004.

5. Clabough EBD and Zeitlin SO: Deletion of the triplet repeat encoding polyglutamine within the mouse Huntington's disease gene results in subtle behavioral/motor phenotypes in vivo and elevated levels of ATP with cellular senescence in vitro. Human Molecular Genetics 15:607-623, 2006.

6. Balan IS, Fiskum G, Hazelton J, Cotto-Cumba C and Rosenthal RE: Oximetry-guided reoxygenation improves neurological outcome after experimental cardiac arrest. Stroke doi:10.1161/01.STR.0000248455.73785.b1, 2006.